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. 2023 Apr 24;51(10):5022–5039. doi: 10.1093/nar/gkad299

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — An integrated quality control response regulates expression of the MMF1 reporter mRNA and protein. (A, B) Expression of the MMF1 protein and mRNA was tested in wild type cells (WT) and in cells lacking HEL2, VMS1, HSP104 or CIS1 growing exponentially by western blotting with antibodies to Flag or with antibodies to Egd2 used as loading control (A) or by RT-qPCR (B). For the mRNA, the levels were normalized to EGD2 and the results are expressed as – ΔCT values in the different strains relative to WT. The level of significant change, relative to WT is indicated with asterisks using a two-sided, Welch, unpaired t-test (n = 3). (C, D) Expression of the MMF1 reporter was tested in WT and atg17Δ cells growing exponentially, before and after a 10 min copper induction for protein (C) and mRNA (D) levels. (E, F) Expression of the MMF1 reporter with or without MTS was tested in WT or in cells lacking HEL2 growing exponentially as in panels A and B, respectively.