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. 2023 Apr 24;51(10):5271–5284. doi: 10.1093/nar/gkad301

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — The DropCRISPRa systems based on variant Cas proteins activate gene transcription. (A, C and E) GFP fluorescence images of condensates in HEK293T cells transfected with the indicated DropCRISPRa (-SpCas9, -AsCas12a and -Un1Cas12f1) systems targeting HBG1. Scale bar, 5 μm. (B, D and F) RT–qPCR revealed relative mRNA expression of HBG1 and IL1RN in HEK293T cells transfected with the indicated systems. Mean values are presented with the SD, n = 3 biological triplicates. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA test versus VP64.