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. 2023 Mar 8;228(5):1153–1176. doi: 10.1007/s00429-023-02619-w

Table 3.

Summary of methods used in studies on the dopaminergic innervation of the primate thalamus

Molecule studied Authors Genus Fixation Postfixation Technique used Plane of cutting Stereotaxic space Thalamic nomenclature Parcellation stains Studied thalamic regions
DA, TH, and DAT Sánchez-González et al. (2005) Human, Macaque Perfusion (PFA or PFA + GTA) Immersion (PFA) [human tissue] IMHQ Coronal YES Olszewski (1952) Nissl, AChE, Myelin Whole thalamus
DA and DAT García-Cabezas et al. (2007) Human, Macaque Perfusion (PFA or MBS + GTA) Immersion (PFA) [human tissue] IMHQ Coronal YES Olszewski (1952) Nissl, AChE, Myelin Whole thalamus
DAT García-Cabezas et al. (2009) Macaque, Rat Perfusion (PFA or PFA + GTA) Immersion (osmium tetroxide) after IMHQ [only for EM] IMHQ Sagittal YES Olszewski (1952) Nissl, AChE, Myelin Whole thalamus
D2 receptors Kessler et al. (1993) Human Unfixed Autoradiography Not specified Not specified Only major subdivisions Not specified Whole brain
D2 and D3 receptors Gurevich and Joyce (1999) Human Immersion (PFA) and unfixed In situ hybridization and autoradiography Not specified Not specified Paxinos (1990) Nissl, AChE Whole brain
D2-like receptors Rieck et al. (2004) Human Immersion (only for NPT assessment) Autoradiography and PET Coronal Not specified Mai et al. (1997) Not specified Whole brain

AChE Acetylcholinesterase, DA dopamine, DAT dopamine transporter, EM electron microscopy, GTA glutaraldehyde, IMHQ immunohistochemistry, MBS sodium metabisulfite, NPT neuropathological, PET positron emission tomography, PFA paraformaldehyde, TH tyrosine hydroxylase