Table 4.
Summary of methods used in studies on the serotoninergic and histaminergic innervation of the primate thalamus
| Molecule studied | Authors | Genus | Fixation | Postfixation | Technique used | Plane of cutting | Stereotaxic space | Thalamic nomenclature | Parcellation stains | Studied thalamic regions |
|---|---|---|---|---|---|---|---|---|---|---|
| Histamine | Lipinski et al. (1973) | Human | Unfixed | – | Enzymatic double-isotope assay | Coronal | Not specified | Only “anterior”, “dorsal” and “pulvinar” | Fresh brain | Whole brain |
| Histamine | Manning et al. (1996) | Macaque | Perfusion (EDCDI or PFA + EDCDI) | Immersion (EDCDI or PFA + EDCDI) | IMHQ | Not specified | Not specified | Kaas et al. (1978). Only LGN and Pul-LP | Not specified | Visual-related structures |
| Histamine and H1, H2, and H3 receptors | Jin et al. (2002) | Human | Immersion (EDAC) | Immersion (PFA) | IMHQ, In situ hybridization, and autoradiography | Coronal | Not specified | Hirai and Jones (1989) | Nissl | Whole thalamus |
| 5-HT | Lavoie and Parent (1991) | Saimiri | Perfusion (PFA + GTA) | Immersion (PFA) | IMHQ | Coronal | Not specified | Adaptation of Emmers and Akert (1963) following Olszewski (1952) | Nissl | Whole thalamus |
| 5-HT1 receptors | Pazos et al. (1987a) | Human | Unfixed | – | Autoradiography | Coronal | Not specified | Dewulf (1971) | Not specified | Whole brain |
| 5-HT2 receptors | Pazos et al. (1987b) | Human | Unfixed | – | Autoradiography | Coronal, horizontal | Not specified | Dewulf (1971) | Not specified | Whole brain |
| 5-HT receptors | Wai et al. (2011) | Human | Immersion (PFA) | – | IMHQ | Coronal | Not specified | Not specified | Nissl, Myelin | Developing thalamus |
5-HT 5-hydroxytriptamine (serotonin), EDAC 1-ethyl-3,3(dimethylaminopropyl)carbodiimide, EDCDI l-ethyl-3(3-dimethylaminopropyl)-carbodiimide, GTA glutaraldehyde, IMHQ immunohistochemistry, LGN lateral geniculate nucleus, LP Lateral posterior nucleus, PFA paraformaldehyde, Pul Pulvinar complex