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. 2023 Jun 8;14:3369. doi: 10.1038/s41467-023-39137-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Insertion of a 200-bp DNA fragment at HEK3 locus by TJ-PE. HEK293T cells were transfected with PE2, nicking sgRNA, and either pegRNA with a control RC-PBS2 (ctrl-RC-PBS2) or a control nicking sgRNA (ctrl-NK) as controls. The insertion band of the predicted size was observed following TJ-PE treatment but not controls (arrow). b Insertion efficiency at HEK3 measured by ddPCR. c Insertion of DNA fragments with PE3 control (pegRNA with a control RC-PBS2 sequence) or TJ-PE at PRNP (left) and IDS (right) loci. Insertion efficiency was measured by ddPCR. Results were obtained from three independent experiments (n = 3), shown as mean ± s.d.