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. 2023 Jun 8;14(6):351. doi: 10.1038/s41419-023-05869-y

Fig. 5. Identification of GADD45A as a C3b/SIN3A complex target gene by genome-wide transcriptional analysis.

Fig. 5

A Genomic distribution of the transcriptional targets of C3b and HDAC1 based on ChIP-Seq data. B DNA binding motifs of C3b and HDAC1 by MEME-ChIP analysis. C Venn diagrams of overlapping genes targeted by C3b and HDAC1 in A549-PTX cells. D Pathway analysis of 1,510 overlapping genes targeted by C3b and HDAC1 in (C). The mRNA levels of the indicated genes as measured by qRT‒PCR in C3-insufficient A549-PTX cells (E) and C3-overexpressing A549-ORI cells (F). The protein levels of C3 and GADD45A are shown in the related figures. The GADD45A mRNA level in A549-ORI and A549-PTX cells measured by qRT‒PCR (G) or RNA-Seq (H). I The binding profiles of C3b and SIN3A on the GADD45A promoter. J Verification of the binding of the C3b/SIN3A complex to the GADD45A promoter by ChIP/qRT‒PCR assay using antibodies against the indicated proteins in A549-PTX cells. Data represent the mean ± SD, n = 3, two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.