Table 4.
Summary of heifer produced chromosomes assemblies. As CLR assembly is better than ONT assembly, we can expect at least similar result after polishing and final steps. For details about pipeline used in this study, refer to Fig. 2.
| ARS-UCD1.2 | ONT wtdbg2 polished | 10X supernova | HiFi hifiasm | |
|---|---|---|---|---|
| Pipeline used | a | b | a | |
| Data type | Hi-C/Optical + Recombination map | Hi-C/10X | 10X Chromium | Hi-C/10X |
| Quantity | 84X Hi-C | 28X/95X | 95X | 28X/95X |
| Scaffolder | HiRise + IrysView | 3D-DNA + Juicer + Juicebox | Supernova | 3D-DNA + Juicer + Juicebox |
| Number of scaffolds | 2 211 | 4 600 | 15 291 | 1 391 |
| Total size | 2 715 853 794 | 2 705 347 253 | 2 663 443 665 | 3 244 660 179 |
| N50 scaffolds length | 103 308 737 | 100 959 810 | 15 206 899 | 87 697 707 |
| BUSCO | C:95.8% | C:95.2% | C:94.7% | C:95.8% |
| Inspector QV | 32.34 | 27.09 | 47.76 |