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. 2023 Mar 17;50(8):2282–2291. doi: 10.1007/s00259-023-06157-9

Fig. 4.

Fig. 4

Difference in ICG loading in a direct chemical comparison between ICG nanocoll and ICG nanoscan. A ICG concentration in nanocoll (blue) and nanoscan fractions (red) based on the area under curve of the absorption curves per fraction, suggesting slightly more ICG binding with ICG nanoscan. B Absorption spectrum measurement of fraction 2 (containing the bulk of the hybrid tracer) of ICG nanocoll (blue line) and ICG nanoscan (red line), suggesting more ICG “stacking” with ICG nanoscan (stacking peak visible around 782 nm). C Fluorescence emission of fraction 2 of ICG nanocoll (blue line) and ICG nanoscan (red line), showing lower emission of ICG nanoscan. Fr fraction number