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. 2023 Jun 8;14:3372. doi: 10.1038/s41467-023-39046-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a LPC-demyelinated lesions of adult mouse corpus callosum analysed at demyelination (3 DPI; days post-injection), oligodendrocyte differentiation/survival (7 DPI), the onset of remyelination (10 DPI) and late remyelination (14–21 DPI). b Reactive (GFAP+; yellow) astrocytes (SOX9+; cyan) in the corpus callosum (outlined) in no-lesion control and at 3, 7 and 14 DPI. Hoechst indicates nuclei in blue. Scale bar, 100 μm. Inset: increased astrocyte hypertrophy over time. Scale bar, 50 μm. c Mean GFAP+ cells/mm² ± s.e.m. Two-way ANOVA with Sidak’s post hoc test versus sham, adjusted P values 0.0142 7 DPI, 0.0164 10 DPI and 0.0057 14 DPI. ANOVA summary (Interaction P = 0.0158, F = 3.338, Time P = 0.0011, F = 5.393, Condition P = 0.0002, F = 17.44). n = 3 mice/group (no lesion, 3, 7, 10, 14, 21 DPI LPC), n = 4 mice/group (7 DPI sham), n = 6 mice/group (3 and 14 DPI sham). d Mean proportion of GFAP + SOX9+ cells/mm² ± s.e.m normalised to total SOX9+ cells. Two-way ANOVA with Sidak’s post hoc test versus sham, adjusted P value 0.0280 3 DPI. ANOVA summary (Interaction P = 0.2851, F = 1.322, Condition P = 0.0004, F = 16.35, Time P value = 0.0331, F = 3.036). n = 3 mice/group (no lesion, 3, 7, 10, 21 DPI LPC), n = 4 mice/group (14 DPI LPC), n = 5 mice/group (7 DPI sham), n = 6 mice/group (3 and 14 DPI sham). e TRAP of lesions in Aldh1l1-EGFP/Rpl10a mice by purification of eGFP-labelled ribosomes from astrocytes. Scale bar, 50 μm. f Volcano plots of adjusted P values against Log2 fold change at 3 DPI, 7 DPI and 10 DPI compared to control (CT), using a threshold of 1.3-fold and adjusted P value of <0.05. Red indicates upregulated genes, blue represents downregulated genes, and grey indicates genes which were not significantly changed. DESeq2 Benjamini–Hochberg test, adjusted P values <0.05. n = 3 mice/group. Source data is provided with this paper. The images in 1a and 1e were created with Biorender.com.