Figure 2.

Analysis of the samples enriched for the specific RIs by agarose gel electrophoresis. An aliquot of the seven samples selected from the preparative gel was loaded (lanes 1–7). To enhance separation of molecules according to their shape, the gel was run under the same electrophoresis conditions as those used for the second dimension of a neutral/neutral 2D gel (see Materials and Methods). After electrophoresis, the gel was transferred and hybridised with the 0.5 kb PvuII fragment of pPI21, used as probe.