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Arf1 positive vesicles are recruited to Lamp1 positive tubule fission sites. Movie relative to Fig. 1 F: Three-dimensional reconstruction of time-lapse fluorescent microscopy images, acquired with a lattice light sheet microscope, of a Lamp1 positive tubule fission event showing recruitment of a Arf1 positive vesicle at the site of fission. MEF cell expressing Arf1-GFP (green) and Lamp1-mCherry (magenta). One frame per second. Frame times are indicated in Fig. 1 F.
