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Arf1 positive vesicles are recruited to sites of autolysosomal tubule fission. Movie relative to Fig. 3 B: Representative time-lapse fluorescent microscopy images of an autolysosomal tubule fission event showing recruitment of an Arf1 positive vesicle at the site of fission. COS7 cell expressing Arf1-GFP (green), Lamp1-SNAP(647) (magenta) and mCherry-LC3 (gray) and incubated in amino acid free media (HBSS) for 8 h. Five frames per second. Frame interval is 2 s.
