Figure 6.

Local intramuscular Dkk3 overexpression abolishes the beneficial effect of MCK-Bc on muscle regeneration. (a) Schematic representation of mouse model using AAV system to achieve local intramuscular GFP or Dkk3 overexpression in WT and MCK-Bc mice and the following experimental setup. (b) TA weight loss in indicated groups at 5 dpi (n = 4–5 mice per group). *P < 0.05, ***P < 0.001; one-way ANOVA with multiple comparisons. (c) Representative immunofluorescence images (left) on Desmin (red), WGA (green), and DAPI (blue), and quantification (right) of the percentage of Desmin positive area percentage of TA muscle cross-sections from indicated groups at 5 dpi. Scale bar: 400 μm. ***P < 0.001; one-way ANOVA with multiple comparisons. (d) Representative immunofluorescence images (left) of laminin (green) and DAPI (blue) and average CSA (right) of TA muscle cross sections from indicated groups (n = 4–5 mice per group; at least three sections/mouse were calculated). *P < 0.05, **P < 0.01; one-way ANOVA with multiple comparisons. Scale bar: 100 μm. (e) Immunoblots of total protein lysates of TA muscles from indicated groups at 5 dpi. All values represent mean ± SEM. All experimental data were verified in at least two independent experiments. Source data are available for this figure: SourceData F6.