Figure S1.

Identification of Baf60c as a potential regulator of muscle regeneration in obesity and T2D. (a) Heatmap representation of the scaled, normalized FPKM (as Z-score) of Baf60s genes in the indicated mouse tissues. The total RNA sample for each tissue was pooled from three C57BL6/J WT mice. Sol, soleus; Gast, gastrocnemius; Quad, quadriceps; TA, tibialis anterior; PL, plantaris; EDL, extensor digitorum longus; Hrt, heart; Dia, diaphragm; WAT, white adipose tissue; BAT, brown adipose tissue. (b) qPCR analysis of Baf60s gene expression (left) and representative immunoblots (right) of total cell lysates during differentiation of C2C12 myotubes (n = 4 biological replicates at each time point). *P < 0.05, ***P < 0.001; one-way ANOVA with multiple comparisons. (c) qPCR analysis of Baf60c gene expression (left; n = 4 mice per group), immunoblots (middle), and quantification of relative Baf60c protein level (right; n = 3 mice per group) of total Quad muscle protein lysates from ob/ob mice and their respective controls. *P < 0.05, **P < 0.01; two-tailed unpaired Student’s t test. (d) qPCR analysis of Baf60c gene expression (left; n = 6 mice per group), immunoblots (middle), and quantification of relative Baf60c protein level (right; n = 3 mice per group) of total Quad muscle protein lysates from db/db mice and their respective controls. **P < 0.01; two-tailed unpaired Student’s t test. (e) Relative BAF60c mRNA levels in human skeletal muscles. For non-diabetic control (CTR), n = 15 independent biological samples; for diabetes, n = 10 independent biological samples. *P < 0.05; two-tailed unpaired Student’s t test. (f) qPCR analysis of Baf60c gene in TA muscle from WT mice during the process of muscle regeneration (n = 3–4 mice at each time point). *P < 0.05, ***P < 0.001; one-way ANOVA with multiple comparisons. All values represent mean ± SEM. All experimental data were verified in at least two independent experiments. Source data are available for this figure: SourceData FS1.