Figure 10.

Influence of the intravesicular pH on the effect of histidylated oligolysine. pRET-Luc cells were incubated with 1 (open bars) or 0.5 µM (hatched bars) GEM-91 and 20 µM HoK2, either in the absence or presence of bafilomycin A₁. The incubation was first carried out for 4 h at 37°C in the presence of 800 nM bafilomycin A₁ and in the presence of GEM-91 and HoK2. Then, the medium was removed and the cells were incubated overnight in fresh complete medium containing 200 nM bafilomycin A₁. Luciferase gene expression was measured by luminescence as described in Materials and Methods. Results show typical experiments carried out in triplicate and repeated at least twice. Data are means ± SD of triplicate experiments.