Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 26;10:1187283. doi: 10.3389/fnut.2023.1187283

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Xi, Zhai, Liu, Gong, Lu, Zhang, Liu, Jin, Zhu, Yin, Xie and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

glut2-deletion results in impaired insulin-mediated anabolic metabolism in MZglut2 fish. (A) Representative images of β-cells in the control and MZglut2 larvae at 5 dpf. (B) The statistical analysis of β-cells in the control (n = 19) and MZglut2 (n = 18) larvae at 5 dpf. (C) Representative images of β-cells in DMSO- and Fisetin-treated larvae at 5 dpf. (D) The statistical analysis of β-cells in DMSO- and Fisetin-treated larvae at 5 dpf (n = 16). (E) Representative images of EdU stained β-cells in the control fish at 5 dpf. Red signal-positive β-cells are indicated by arrows (n = 8). (F) Representative images of EdU staining β-cells in MZglut2 larvae at 5 dpf (n = 8). (G) WISH using the insulin probe to the control and MZglut2 larvae at 5 dpf, with the green arrow representing the signal area. (H) The transcriptional expression levels of insra, insrb, chrebp, srebf1, and srebf2 in the liver of the control and MZglut2 fish at 3 mpf (n = 6). (I) The transcriptional expression levels of aclya, fasn, acc, fads2, scd, dgat1a, and dgat2 in the liver of the control and MZglut2 fish at 3 mpf (n = 6). (J) The transcriptional expression levels of atgl, lpl, pparab, cpt1aa, cpt1ab, and acox3 in the liver of the control and MZglut2 fish at 3 mpf (n = 6). (K) The quantification of carnitine metabolites based on the observations of metabolomics in the liver of control and MZglut2 fish at 3 mpf (n = 6). (L) The measurement of the lipid content of the control (n = 9) and MZglut2 (n = 8) fish at 3 mpf. (M) Nile Red staining of the control and MZglut2 fish at 3 mpf. White arrows: visceral adipose tissue. (N) The bar chart represents the relative fluorescence intensity of the control and MZglut2 fish after Nile Red staining (n = 8). (O) Liver triglyceride content at 3 mpf (n = 6). (P) Oil Red O staining of liver sections for distribution of lipid droplets in the control and MZglut2 fish at 3 mpf. (Q) The bar chart represents the relative lipid droplet areas of control and MZglut2 fish after Oil Red O staining (n = 8). A two-tailed unpaired t-test was used to detect significance. ****P < 0.0001.***P < 0.001. **P < 0.01. *P < 0.05. ns, no significance.