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. 2023 Jun 8;6(8):e202301957. doi: 10.26508/lsa.202301957

Figure 4. Expression levels of cytokines in the circulation of PE mother and NFκB phosphorylation in the cortices of their embryos at E17.5.

Figure 4.

(A) Mouse Th1/Th2/Th17 array showing multiple cytokines, including IL-4, TNFα, IL-17, IL-2, IL-28, IFN-γ, MIP-3a, IL-1b, IL-1 p70, IL-5, IL-6, IL-10, IL-17F, IL-23, IL-13, IL-21, TNFβ1, and IL-22, in maternal serum (upper panels), fetal cortex (middle panels), and concentration of total serum protein (down panel) at pregnancy day (PD) 17.5 (N = 6 fetuses from six dams for each group). (B) qRT-PCR analysis of the expression of Tnfaip3 in the placenta on PD17.5 from control and PE groups. (n = 2–3 placentas per dam, N = 6 dams per group). (C, D) Representative Western blotting images (C) and a graph (D) showing overactivation of NFκB in the cortices of embryos from PE mothers on E17.5 (n = 6 fetal cortexes from three dams per group). Data are presented as the mean ± s.e.m. NS, P ≥ 0.05; *, P < 0.05; ***, P < 0.001, versus control. (A, D) Multiple t tests with the Holm–Sidak correction for panels (A, D). (B) Unpaired, two-tailed test for panel (B).