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. 2023 Jun 8;6(8):e202301957. doi: 10.26508/lsa.202301957

Figure S8. Effects of TNFα neutralization in maternal serum on anxiety and social behavior in offspring exposed to PE.

Figure S8.

(A, B, C) Time spent in the closed arm of the elevated plus maze (n = 20, 16, 20, and 20 male offspring from 4–5 litters and 20, 16, 20, and 16 female offspring from 4–5 litters for control+IgG, control+TNFα antibody, PE+IgG, and PE+TNFα antibody groups, respectively); (B) social quotient, calculated as (time spent in the social chamber - time spent in the non-social chamber)/time spent in the middle chamber, in the three-chamber test (n = 16 offspring from 4 litters per sex per group); (C) total travel distance in the open-field test (n = 20 male offspring from 5 litters per group; n = 24, 20, 24, and 16 female offspring from 4–6 litters for control+IgG, control+TNFα antibody, PE+IgG, and PE+TNFα antibody groups, respectively). Data are presented as the mean ± s.e.m. NS, P ≥ 0.05; **, P < 0.01; ***, P < 0.001, versus control+IgG. One-way ANOVA, Tukey’s HSD.