Effects of metformin on PTM, lumbar vertebrae and bone biomechanical properties in GK diabetic rats (A) Representative micrographs of PTM histomorphometry (undecalcified sections with Goldner trichrome staining) (B) Effects of metformin on static parameters of right upper tibia cancellous bone in GK rats (C) Representative micrographs of lumbar vertebrae (undecalcified sections with Goldner trichrome staining). Effects of metformin on (D) static parameters of lumbar cancellous bone and (E) biomechanical properties of bone in GK rats. Note: Vs. Control ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; vs. GK #P < 0.05, ##P < 0.01, ###P < 0.001. Values are presented as mean ± SD.