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. 2023 Jun 3;40:37–48. doi: 10.1016/j.jot.2023.05.002

Fig. 7.

Fig. 7

Effects of metformin on osteogenic differentiation of C3H10 ​cells induced with high concentrations of glucose (A) Chemical structural of metformin (B) Wayne diagram of potential targets of metformin in the treatment of T2DM-OP disease (C) Protein interaction network of potential targets (D) Degree values of top ten potential targets (E) Gene expression of C3H10 ​cells induced by high glucose visualized in KEGG pathway of the RAGE-JAK2-STAT1 signal axis (F) RAGE, STAT1, RUNX2, Col1a1, OCN and ALP gene expression in C3H10 ​cells induced by high glucose determined by RT-qPCR (G) Western blots show effects of metformin on protein levels of RAGE, p-JAK2, JAK2, p-STAT1, STAT1 and Osterix in C3H10 ​cells induced by high glucose (H) Protein levels of RAGE, p-JAK2/JAK2, p-STAT1/STAT1 and Osterix. Note: Vs. Control ∗P ​< ​0.05, ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001; vs. OIM #P ​< ​0.05, ##P ​< ​0.01, ###P ​< ​0.001; vs. OIM ​+ ​GH △P ​< ​0.05, △△P ​< ​0.01, △△△P ​< ​0.001. Values are presented as mean ​± ​SD.