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. 2023 May 19;26(6):106899. doi: 10.1016/j.isci.2023.106899
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit monoclonal [EPR3610] to Ki67 Abcam Cat#ab92742; RRID:AB_10562976
Rabbit polyclonal to Cleaved Caspase-3 (Asp175) Cell Signaling Technology Cat#9661; RRID:AB_2341188
Goat polyclonal anti-rabbit IgG Agilent Cat#E0432; RRID:AB_2313609
Goat polyclonal anti-rabbit IgG Vector Laboratories Cat#PK-4001; RRID:AB_2336810
Biological samples
Primary human PDAC cells from PaCaOmics cohort Gayet et al.53 N/A
Chemicals, peptides, and recombinant proteins
Serum-free ductal media (SFDM) Gayet et al.53 N/A
Gemcitabine (Gemzar) Eli Lilly & Co. N/A
Etomoxir sodium salt hydrate Sigma-Aldrich Cat#E1905
Perhexiline maleate salt Sigma-Aldrich Cat#SML0120
Trimetazidine dihydrochloride Sigma-Aldrich Cat#653322
Sodium octanoate Sigma-Aldrich Cat#C5038
Sodium acetate Sigma-Aldrich Cat#S2889
Critical commercial assays
Seahorse XF Cell Mito Stress Test Kit Agilent Technologies Cat#103015-100
Seahorse XF Mito Fuel Flex Test Agilent Technologies Cat#103260-100
Pacific Blue™ Annexin V Apoptosis Detection Kit with PI BioLegend Cat#640928
BODIPY™ 493/503 Invitrogen™ Cat#D3922
BODIPY™ 581/591 C11 (Lipid Peroxidation Sensor) Invitrogen™ Cat#D3861
CellRox Orange Invitrogen™ Cat#C10493
MitoSOX Red Invitrogen™ Cat#M36008
Trichrome Stain Kit (Connective Tissue Stain) Abcam Cat#ab150686
VECTASTAIN® ABC-HRP Kit, Peroxidase (Rabbit IgG) Vector Laboratories Cat#PK-4001
RNeasy Mini Kit Qiagen Cat#74104
GoScript™ Reverse Transcription System Promega Cat#A5001
Deposited data
Original and analyzed data This paper Mendeley data: https://doi.org/10.17632/8yg3837dsc.1
PaCaOmics RNA-seq data Nicolle et al.54 Accession number E-MTAB-5039
PaCaOmics metabolome data Kaoutari et al.36 N/A
Experimental models: Cell lines
Panc-1 ATCC Cat#CRL-1469
Experimental models: Organisms/strains
Mouse: Female Swiss nude, SOPF (4 weeks old) Charles River Cat#Crl:Nu(lco)-Foxn1nu
Oligonucleotides
CPT1A Forward CCA GAC GAA GAA CGT GGT CA Brown et al.58 N/A
CPT1A Reverse ATC TTG CCG TGC TCA GTG AA Brown et al.58 N/A
CPT1B Forward CTG GGC TAT GTG TAT CCG CC Brown et al.58 N/A
CPT1B Reverse GCA CAG ACT CTA GGT ACC GC Brown et al.58 N/A
CPT1C Forward TTT GCC TCG TGT TTG TGG G Zhang et al.59 N/A
CPT1C Reverse CAG CCG TGG TAG GAC AGA A Zhang et al.59 N/A
CPT2 Forward GTA GCA CTG CCG CAT TCA AG Brown et al.58 N/A
CPT2 Reverse GCC ATG GTA CTT GGA GCA CT Brown et al.,58 N/A
CPT1C siRNA, ON-TARGETplus siRNA Reagents Dharmacon Cat# L-008824-01-0020
siRNA pool, ON-TARGETplus Non-targeting Pool Dharmacon Cat# D-001810-10-20
INTERFERin® transfection reagent Polyplus-transfection Cat# POL101000028
Software and algorithms
Fiji ImageJ https://imagej.nih.gov/ij N/A
CALOPIX digital software Tribun Health N/A
FlowJo version 10.0.7 https://www.flowjo.com/solutions/flowjo N/A
GraphPad Prism https://www.graphpad.com/ N/A