Figure 1.

The antisense strand of box 2 of the VSG promoter binds the same factor as the sense strand of box 1. Bandshift assays were carried out with the indicated oligonucleotide probes, in the presence of increasing amounts (5-, 50- and 500-fold molar excess) of the indicated cold oligonucleotides. The assays were performed with nuclear extracts from bloodstream forms except in lane 16 (PF, procyclic forms). Complexes are numbered with roman numbers. The specific complex (band II) is arrowed.