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. 2000 Jan 15;28(2):582–592. doi: 10.1093/nar/28.2.582

Figure 6.

Figure 6

Shuttling of P=S ODNs is less sensitive to nuclear RanGTP depletion than classical protein export. (A) Co-injection of 30 µM P=S ODN 12182–Texas Red and 1.7 mg/ml RanT24N into one nucleus of a binucleate Ref52 cell. Microscopic observation of the fixed cell 2 h after injection indicated that shuttling was only partly inhibited. (B) In a control co-injection of 12182–Texas Red and 1.7 mg/ml wild-type Ran protein shuttling proceeded normally. (C) The same preparation of 1.7 mg/ml RanT24N protein when co-injected with 2 mg/ml export substrate GGNES abolished protein export. The reporter protein was still nuclear 1 h after injection. (D) Co-injection of 2 mg/ml export substrate GGNES with an excess of 2 mM P=S ODN 8424 indicated that GGNES was normally exported within 1 h. The arrowheads in (A) and (B) point to the non-injected nuclei. Bars 10 µm.