Table 3.
Sequences of linkers, HA epitopes, URA3 or RPL15A promoters, and TRP1 terminator
| Sequence type | Amino acid sequence (N → C) | Nucleotide sequence (5′ → 3′) |
|---|---|---|
| GOI-HA-linker | SGSASGGGGSGG | AGTGGGTCTGCTAGCGGTGGAGGTGGGAGTGGAGGC |
| HA sequence | YPYDVPDYA | TATCCTTACGACGTCCCCGATTACGCA |
| TATCCGTATGACGTACCCGATTATGCT | ||
| TACCCATACGACGTTCCCGACTACGCT | ||
| TATCCGTATGATGTGCCAGACTATGCA | ||
| TATCCATATGATGTTCCTGATTACGCC | ||
| TACCCCTATGATGTACCAGACTATGCG | ||
| Linker between HA sequences | GGSGGSGG | GGTGGCAGTGGGGGGTCAGGCGGA |
| GGGSGGSG | GGAGGTGGTTCAGGTGGCTCAGGA | |
| GGGSGGSG | GGTGGCGGCTCTGGTGGCTCAGGT | |
| GGSGGSGG | GGAGGTTCCGGCGGTAGCGGAGGC | |
| GSGGGGSG | GGCTCAGGAGGGGGCGGAAGCGGT | |
| URA3 promoter | – | -175 GTTTCTTTGA….AAGATAAATC -1 |
| RPL15A promoter | – | -623 GTATTCAAGA…TAAATCAGCA -1 |
| TRP1 terminator | – | GTTATTACTGAGTAGTATTTATTTAAGTATTGTTTGTGCACTTGCCT |