Table 4.
Yeast transformation reaction components
| Reagent | Volume |
|---|---|
| PEG 3350 50% | 480 μL |
| LiAc 1 M | 72 μL |
| Boiled Herring sperm DNA | 10 μL |
| Digested plasmid | 1–2 μg (25–50 μL) |
| Yeast cells | ≈50–100 μL |
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