Table 5.
Primers sequence for validation of 2E2-FP and HA cassettes integration in the yeast genome
| Name | Sequence (5′ → 3′) | Description |
|---|---|---|
| KanMX Rev | CAGCATCCATGTTGGAATTTAATCGCGG | Validation of kanMX integration |
| NatMX Frw | GCGCTCTACATGAGCATGCCCTGC | Validation of natMX integration |
| HphMX Frw | GCTCTCGATGAGCTGATGCTTTGG | Validation of hphMX integration |
| HphMX Rev | CATCAGGTCGGAGACGCTGTC | |
| HphMX Rev2 | ATCGGCGAGTACTTCTACACAGCC | |
| TEFterm Frw | GCTGGTCGCTATACTGCTGTCG | Validation of TEFterm integration |
| TEFterm Rev | CGAATCGACAGCAGTATAGCGACCAGC | |
| Ade1Locus Frw | CAGGCCTTCCACTTTTGAATTACTGC | Validation of 2E2-FP integration in ADE1 locus |
| Ade1Locus Frw2 | AGCGAGCCAGGGAAGTAATTAGCG | |
| Ade1Locus Rev | AACTCCTTAAGTACTACCACAGCCAG | |
| Ade1Locus Rev2 | GTCTGACTCTTGCGAGAGATGAGG |