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. 2023 Jun 5;18(1):20230730. doi: 10.1515/med-2023-0730

Figure 2.

Figure 2

Midazolam retarded cell proliferation and migration and promoted cell apoptosis in SK-MES-1 and A549 cells. (a) After SK-MES-1 and A549 cells were treated with 100, 200, or 400 μM midazolam, cell proliferation was detected by EdU staining assay. (b) Colony formation assay was carried out to determine the colony-forming capacity of SK-MES-1 and A549 cells exposed to 100, 200, or 400 μM midazolam. (c) Flow cytometry scatter diagram under the midazolam treatment. The role of midazolam in the (d) necrosis and (e) early apoptosis of SK-MES-1 and A549 cells was estimated by flow cytometry analysis. (f) Transwell assay was adopted to evaluate the influences of midazolam on cell migration in NSCLC. All results were represented as mean ± SD and each assay was conducted in triplicate. * P  <  0.05, ** P  <  0.01, *** P  <  0.001.