| 1 |
Roots/root nodule of Arachis hypogea |
Endophytic bacteria/bacterioids |
Microflex, (Bruker Daltonics Inc.) |
10 mg mL−1 HCCA in 50% ACN, 2.5% TFA |
Place a small portion of a single colony directly onto a clean MALDI target plate and overlay 1 μL of matrix on the sample. Analyze the sample in MALDI MS after it gets complete dryness at room temperature |
Biotyper 1.0 software |
Strains of Rhizobium sp. |
Manikandan et al.59
|
| 2 |
Root nodules from G. max, M. atropurpureum and V. unguiculata
|
Endophytic bacteria/bacterioids |
Axima™ Confidence machine (Shimadzu-Biotech Corp.) |
A saturated solution of CHCA in 33% acetonitrile, 33% ethanol, and 3% TFA |
(i) Direct smear method, (ii) on spot extraction with 25% formic acid, and (iii) extraction with ACN and formic acid (70%). Overlaying 1 μL of matrix on samples |
SARAMIS™ Superspectrum tool |
Strains of Sinorhizobiumgenus
|
Ziegler et al.96
|
| 3 |
Leaves of tropical palm, Astrocaryum sciophilum
|
Endophytic fungi |
UltrafleXtreme mass spectrometer (Bruker Daltonics, Inc.) |
CHCA matrix (50 mg mL−1 in ACN/H2O (50/50) containing 0.1% TFA) |
Washing solution (300 μL of water and 900 μL of 100% ethanol), centrifugation (13 000 rpm). Resuspend the pellet in 40 μL of formic acid and 40 μL 100% acetonitrile. Use the supernatant after centrifugation at 13 000 rpm, as a sample. Overlaying 1 μL of CHCA on the sample spot |
Biotyper 3.0 software |
Colletotrichum strains |
Barthelemy et al.97
|
| 4 |
Garlic roots, stem leaves |
Endophytic bacteria |
UltrafleXtreme mass spectrometer (Bruker Daltonics, Inc.) |
CHCA matrix (50 mg mL−1 in ACN/H2O (50/50) containing 0.1% TFA) |
Bruker standard sample preparation protocol |
Biotyper 3.0 software |
Strains of Bacillus subtilis, B. marisflavi, B. koreensis, B. megaterium, Enterobacter cloacae, Pantoea dispersa, Rhizobium radiobacter, Paenibacillus massiliensis, Lactobacillus paracasei, Burkholderia cepacian, Azospirillum brasilense
|
Júnior et al.93
|
| 5 |
Great Nettle (Urtica dioica L.) stems, leaves and roots |
Endophytic bacteria |
Microflex LT MALDI-TOF mass spectrometer (Bruker Daltonics Inc.) |
CHCA matrix (saturated solution of CHCA in 500 μL of 50% (v/v) ACN, 250 μL of 10% (v/v) TFA) and 250 μL of H2O) |
Smearing of bacterial colony and addition of 1.5 μL of the matrix solution |
Biotyper 3.0 software |
Strains of Bacillus anthracis, B. megaterium, B. pumilus, B. cereus, Escherichia coli, Pantoea agglomerans, Enterobacter amnigenus, Staphylococcus cohnii, Enterococcus faecium, Paenibacillus lautus, P. glucanolyticu
|
Toubal et al.94
|
| 6 |
Curcuma zedoaria
|
Endophytic bacteria |
MALDI-TOF MS Axima system (Shimadzu-Biotech Corp.) |
CHCA matrix 1 μL each of CHCA: ethanol : ACN : water : TFA |
Bacterial cells lysed in 20 μL formic acid (25% v/v). From the supernatant 1 μL matrix solution overlay on samples |
SARAMIS software |
Strains of Alcaligenes faecalis, Bacillus subtilis, Enterobacter aerogenes, Enterobacter, Klebsiella pneumoniae, Pantoea dispersa, Pseudomonas stutzeri, Stenotrophomonas maltophilia
|
Sulistiyani and Lisdiyanti, 2018 |
| 7 |
Wheat roots (Triticum vulgare) |
Endophytic bacteria |
Autoflex II MALDI-TOF (Bruker Daltonics Inc.) |
CHCA matrix (10 mg mL−1) CHCA 10 mg in ACN (50% v/v) and TFA (2.5% v/v) |
Bacterial colonies on a plate and 1 μL matrix solution overlaying on samples. 40 μL of a formic acid (35%) : acetonitrile (50%) mixture and centrifuged at 25 000g for 15 min |
PECLUST (Alm et al., 2006) |
Clustering of bacterial isolates. Strains of Pantoea strains, Pseudomonas, Enterobacter, Acetnobacter
|
Stets et al.89
|
| 8 |
Canola Roots (Brassica napus L. var. oleracea) |
Endophytic bacteria |
Autoflex III MALDI-TOF/TOF mass spectrometer (Bruker Daltonics, Inc.) |
CHCA (10 mg mL−1) in ACN (50% v/v) and TFA (2.5% v/v) |
Washing solution (300 μL of water and 900 μL of 100% ethanol), centrifugation (13 000 rpm). Resuspend the pellet in 40 μL of formic acid and 40 μL 100% acetonitrile. Use the supernatant after centrifugation at 13 000 rpm, as a sample. Overlaying 1 μL of CHCA on the sample spot |
MALDI Biotyper 3.0 software |
Strains of Bacillus subtilis, B. simplex, B. niacin, P. amylolyticus, P. glucanolyticus, P. massiliensis, P. polymyxa, Pseudomonas koreensis, P. thivervalensis
|
Martínez-Hidalgo et al.
95
|
| 9 |
Roots, stems, leaves of Astragalus bisulcatus and Stanleya pinnata
|
Endophytic bacteria |
Biflex IV MALDI-TOF spectrometer (Bruker Daltonics, Inc.) |
CHCA matrix (10 mg mL−1) in ACN (50% v/v) and TFA (2.5% v/v) |
Smearing of the bacterial colony and addition of 1.0 μL of the matrix solution |
Biotyper 2.0 software |
Strains of Bacillus, Pseudomonas, Pantoea, Staphylococcus, Paenibacillus, Advenella, Arthrobacter, and Variovorax
|
Jong et al.92
|
| 10 |
Pear slices, and necrotic pear plant tissueapple flowers |
Pathogenic bactera |
Microflex LT MALDI-TOF mass spectrometer (Bruker Daltonics Inc.) |
CHCA matrix (10 mg mL−1) in ACN (50% v/v) and TFA (2.5% v/v) |
Bacteria grown in liquid medium, harvested by centrifugation, washed with water resuspending in 0–3 mL water and 0–8 mL ethanol, lysed with 40 μL of 70% formic acid and 40 μL acetonitrile. Supernatant used as samples |
Biotyper 2.0 software |
Erwinia amylovora and Erwinia pyrifoliae, Erwinia tasmaniensis and Erwinia billingiae
|
Wensing et al.115
|
| 11 |
Specimens of intact bunt balls, mainly in the ears of wheat plant |
Pathogenic fungi |
Microflex LT MALDI-TOF mass spectrometer (Bruker Daltonics Inc.) |
CHCA matrix (10 mg mL−1) in ACN (50% v/v) and TFA (2.5% v/v) |
Mycelium/teliospores were suspended in 100 μL of formic acid (70%) and lysed by bead beating and homogenization addition of 100 μL of ACN (100%) and centrifuged at 20 000g. Supernatant used as samples |
Biotyper 3.0 software |
Tilletia controversa, T. caries, T. laevis
|
Forster et al.114
|
| 12 |
Citrus leaves (healthy and infected) |
Pathogenic bacteria causing citrus cancer |
Autoflex speed spectrometer (Bruker Daltonics, Inc.) |
CHCA matrix (20 mg mL−1) in acetonitrile 0.2% aqueous trifluoroacetic acid (1 : 1) |
Spotted samples overlaid by 1.5 μL of the matrix solution |
Biotyper 3.1 software |
Xanthomonas citri subsp. citri |
Niculau et al.111
|
| 13 |
Quinoa (Chenopodium quinoa) |
Pathogenic bacteria causing leaf spot |
Microflex MALDI-TOF mass spectrometer (Bruker Daltonics Inc.) |
CHCA matrix (10 mg mL−1) in ACN (50% v/v) and TFA (2.5% v/v) |
Smearing bacteria on MALDI MS target plate 1 μL of formic acid and analyzed with 1 μL of organic matrix CHCA |
Biotyper 3.1 software |
Pseudomonas syringae
|
Fonseca-Guerra et al.106
|
| 14 |
Miscanthus sinensis |
Pathogenic bacteria causing leaf blight |
Instrument information not provided |
Matrix information not provided |
The methodology is not provided |
Biotyper 3.0 software |
Pseudomonas syringae pv. syringae |
Choi et al.108
|
| 15 |
Rice seedlings |
Pathogenic bacteria causing panicle blight, and foot rot |
Ultraflex MALDI-TOF mass spectrometer (Bruker Daltonics Inc.) |
CHCA matrix (10 mg mL−1) in ACN (50% v/v) and TFA (2.5% v/v) |
Bacteria treated with 70% (v/v) formic acid and the same volume of acetonitrile, and centrifuged at 15 000 rpm for 5 min and used for the analysis with 1 μL of organic matrix CHCA |
Biotyper 3.1 software |
Burkholderia glumae, Burkholderia gladioli pv. gladioli, and Erwinia chrysanthemi pv. zeae |
Kajiwara105
|
| 16 |
Pathogenic cultures |
Pathogenic bacteria causing blackleg and soft rot diseases |
4700 Proteomics Analyzer (Applied Biosystems, Inc.) |
Sinapinic acid matrix solution (16 mg mL−1 in ACN/0.1% TFA, 3 : 2, v/v) in 1 : 1 ratio |
Bacteria treated with EtOH and then with ACN/FA solution (ACN/5% FA, 1 : 1, v/v). The final bacterial suspension was mixed with SA matrix solution and overlaid with the ACN/H2O solution (ACN/H2O, 3 : 2, v/v) |
Information regarding spectral analysis not provided |
Dickeya and Pectobacterium species |
Šalplachta et al.104
|
| 17 |
Damaged plants of different ornamental, forest and fruit woody plant species |
Pathogenic fungi (oomycetes) |
Autoflex speed MALDI-TOF mass spectrometer (Bruker Daltonics Inc.) |
CHCA matrix solution CHCA 10 mg mL−1 (acetonitrile 50%, water 47.5% and trifluoracetic acid 2.5%) |
Washing solution (300 μL of water and 900 μL of 100% ethanol), centrifugation (13 000 rpm). Resuspend the pellet in 40 μL of formic acid and 40 μL 100% acetonitrile. Use the supernatant after centrifugation at 13 000 rpm, as a sample. Overlaying 1 μL of CHCA on the sample spot |
Biotyper 3.1 software |
Strains in genus Phytophthora and Pythium
|
Bozik et al.110
|
| 18 |
Rice seedlings |
Pathogenic bacteria causing brown stripe on rice |
Autoflex MALDI-TOF mass spectrometer (Bruker Daltonics Inc.) |
CHCA matrix solution CHCA 10 mg mL−1 (acetonitrile 50%, water 47.5% and trifluoracetic acid 2.5%) |
Washing solution (300 μL of water and 900 μL of 100% ethanol), centrifugation (13 000 rpm). Resuspend the pellet in 40 μL of formic acid and 40 μL 100% acetonitrile. Use the supernatant after centrifugation at 13 000 rpm, as a sample. Overlaying 1 μL of CHCA on the sample spot |
Information regarding spectral analysis not provided |
Acidovorax oryzae and Acidovorax citrulli
|
Wang et al.100
|
| 19 |
Isolated from different plant parts |
Pathogen causing diseases in alfalfa, tomato, corn, potato and wheat |
4800 Plus MALDI TOF/TOFTM Analyzer (AB Sciex Inc.) |
CHCA matrix solution CHCA 10 mg mL−1 (acetonitrile 50%, water 48% and trifluoracetic acid 2%) |
Washing solution (300 μL of water and 900 μL of 100% ethanol), centrifugation (18 000 rpm). Resuspend the pellet in 50 μL of formic acid and 50 μL 100% acetonitrile. Use the supernatant after centrifugation at 18 000 rpm, as a sample. Overlaying 1 μL of CHCA on the sample spot |
BioNumerics 5.1 software (Applied Maths, Belgium) |
Clavibacter strains |
Zaluga et al.99
|
| 20 |
Isolated from leaves and roots |
Endophytic bacteria of banana plant |
Autoflex speed MALDI-TOF mass spectrometer (Bruker Daltonics Inc.) |
Followed manufacturers' instruction |
Followed manufacturers' instruction |
Biotyper 4.1.60. software |
Enterobacter cloacae
|
Macedo-Raygoza et al.102
|
