The average hydrodynamic diameter (dh) and zeta potential (ζ) of silver nanoparticles (AgNPs), stabilized with sodium bis(2-ethylhexyl) sulfosuccinate (AOT-AgNPs), poly(vinylpyrrolidone) (PVP-AgNPs), and citrate (cit-AgNPs) suspended in anionic reactant solution (c(Na2HPO4) = 8 × 10−3 mol dm−3, γ (AgNPs) = 20 mg dm−3, pH 7.4) in the presence or absence of bovine serum albumin (BSA, γ = 200 mg dm−3) and chitosan (Chi, γ = 50 mg dm−3) at 25 °C.
| Sample | Peak I | Peak II | Peak III | ζ/mV | |||
|---|---|---|---|---|---|---|---|
| d h/nm | vol% | d h/nm | vol% | d h/nm | vol% | ||
| Anionic reactant solution | |||||||
| AOT-AgNPs | 88.1 ± 1.2 | 100 | −47.3 ± 1.7 | ||||
| PVP-AgNPs | 84.1 ± 1.9 | 100 | −18.2 ± 3.5 | ||||
| cit-AgNPs | 20.7 ± 6.1 | 73.3 ± 7.1 | 85.2 ± 4.2 | 26.8 ± 7.1 | −46.7 ± 2.6 | ||
| BSA | 6.6 ± 0.2 | 100 | −8.4 ± 2.9 | ||||
| Chi | 91.5 ± 14.2 | 100 | 4.1 ± 1.5 | ||||
| Anionic reactant solution with BSA | |||||||
| AOT-AgNPs | 101.7 ± 1.1 | 100 | −25.1 ± 3.7 | ||||
| PVP-AgNPs | 100.6 ± 1.3 | 100 | −16.0 ± 7.4 | ||||
| cit-AgNPs | 103.3 ± 5.7 | 100 | −28.7 ± 1.7 | ||||
| Anionic reactant solution with Chi | |||||||
| AOT-AgNPs | 154.5 ± 2.4 | 100 | 2.7 ± 2.7 | ||||
| PVP-AgNPs | 161.7 ± 12.0 | 26.0 ± 2.2 | 1120.8 ± 38.6 | 57.0 ± 3.5 | 4385.3 ± 161.1 | 17.1 ± 1.8 | 0.0 ± 2.1 |
| cit-AgNPs | 136.4 ± 2.5 | 100 | 2.0 ± 2.4 | ||||