Figure 2.

Conversion of the intramolecular transplatin intrastrand crosslink within OMeFSPt. The platinated oligonucleotide has been incubated under the conditions described in Materials and Methods either with 53FS or 28FS RNA. (a) Kinetic of the cross-linking reaction between OMeFSPt and 53FS (top) or 28FS (bottom) monitored by electrophoresis on a denaturing polyacrylamide gel (RNAs were ³²P 5′ end-labelled). The OMeFSPt–53FS RNA adduct is indicated by an arrowhead to the right. The incubation time is given at the top of each lane. (b) Autoradiographs shown in (a) were quantitated by INSTANTIMAGER analysis and the crosslinked RNA was plotted versus time for 53FS (circle) and 28FS RNA (square).