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Determination of the Pt-crosslinked residues on the target RNA. 32P 5′ end-labelled 53FS RNA alone (lane 2), mixed with OMeFSPt (lane 3) or with OMeFS (lane 4) was submitted to alkaline hydrolysis and run on a denaturing polyacrylamide gel. Lane 1 corresponds to the RNase T1 digest of 53FS RNA which was used to determine the RNA sequence given to the right.
