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. 2023 Jun 9;13:9403. doi: 10.1038/s41598-023-36428-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Determining the ability of modified aptamers to distinguish between RSV’s prefusion and postfusion F protein by AlphaScreen. Biotin labelled aptamers were mixed with either the prefusion or postfusion form of F protein and Palivizumab. Relative AlphaScreen signal was calculated by forming a ratio of the sample fluorescence and the aptamer-free background fluorescence. The increased values indicate selective binding of aptamers to the different F protein forms. Error bars represent the standard deviation of three technical replicates.