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. 2023 May 29;50(1):138. doi: 10.3892/or.2023.8575

Figure 2.

Figure 2.

circ_0024107 mediates the promotion of gastric cancer cell metastasis by GC-MSCs in vitro and in vivo. (A) Schematic diagram of the specific siRNAs targeting the back-splicing site of circ_0024107. (B) RT-qPCR of circ_0024107 in GC-MSCs following transfection with two different siRNAs against circ_0024107. NC was the negative control of the two siRNAs. (C-E) Transwell assays of the migration and invasion of gastric cancer cell lines, AGS and HGC-27, following treatment with CM from circ_0024107-silencing GC-MSCs. Scale bars, 100 µM; magnification, ×200. (F) Schematic diagram of circ_0024107 overexpression vector. (G) RT-qPCR of circ_0024107 in BM-MSCs following transfection with the overexpression vector. (H-J) Migration and invasion analysis of gastric cancer cells following treatment with CM from circ_0024107-overexpressing BM-MSCs. Scale bars, 100 µM; magnification, ×200. (K-M) Lymphatic metastasis of HGC-27 treated with CM from circ_0024107-silenced GC-MSCs in vivo. (K) Lymph nodes; (L) weight of lymph nodes; (M) representative images of pan-cytokeratin AE1/AE3 staining in lymph nodes. Magnification, ×100 and scale bars, 100 µM; or magnification, ×400 and scale bars, 20 µM. Values are presented as the mean ± SD (n=3) *P<0.05, **P<0.01 and ***P<0.001, vs. respective control. circRNA, circular RNA; GC-MSCs, gastric cancer-derived mesenchymal stem cells; BM-MSCs, bone marrow-derived mesenchymal stem cells; RT-qPCR, reverse transcription-quantitative PCR; CM, conditioned medium.