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. 2023 May 29;50(1):138. doi: 10.3892/or.2023.8575

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — miR-5572 and miR-6855-5p reverse the circ_0024107-induced FAO reprograming of GC-MSCs. (A-D) CPT1 activity and β-oxidation rate assay in (A and B) miRNA mimic-transfected GC-MSCs and (C and D) inhibitor-transfected BM-MSCs. (E and F) Count of the migrated and invaded gastric cancer cells following incubation with CM from (E) miRNA mimic-transfected GC-MSCs and (F) inhibitor-transfected BM-MSCs. (G) Count of the migrated gastric cancer cells following incubation with CM from BM-MSCs in which the two miRNAs were silenced and which were treated with etomoxir. Detection of (H) CPT1A mRNA and (I) protein levels in BM-MSCs co-transfected with circ_0024107 overexpression vector and miRNA mimics. Measurement of FAO activity assay by determining (J) CPT1 activity and (K) β-oxidation rate in the co-transfected BM-MSCs. (L and M) Transwell assay of the migration and invasion of gastric cancer cells following incubation with CM from the co-transfected BM-MSCs. Scale bars, 100 µM; magnification, ×200. Values are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001, vs. respective control. circRNA, circular RNA; GC-MSCs, gastric cancer-derived mesenchymal stem cells; BM-MSCs, bone marrow-derived mesenchymal stem cells; CPT1A, carnitine palmitoyltransferase 1A; CM, conditioned medium; FAO, fatty acid oxidation; ETO, etomoxir.