Figure 5.

GST-Z2 binds the DSR more avidly than does GST-Z1. GST-Z1 and GST-Z2 were bound to labeled DSR probe at room temperature under standard gel shift conditions. After 20 min a 1000-fold molar excess of unlabeled probe was added, the incubation was continued and samples were withdrawn at the indicated time points and loaded directly onto gels as described in the Materials and Methods. (A) Autoradiograph of GST-Z1 binding after addition of unlabeled probe (time 0). (B) Autoradiograph of GST-Z2 binding after addition of unlabeled probe. (C) Quantification of percent probe bound as a function of time after the addition of unlabeled probe.