Table 2.

Intrinsic Phosphodianion Binding Energies for Wildtype and Variant Forms of OMPDC and the Effects of these Amino Acid Substitutions on the Intrinsic Phosphodianion Binding Energy $△{\text{G}}_{\text{Pi}}^{‡}$ (eq 1).^a

OMPDC	$\frac{{\text{(}{k}_{\text{cat}}\text{/}{K}_{\text{m}}\text{ )}}_{\text{S}-\text{Pi}}}{k_{\text{cat}}\text{/}{K}_{\text{SH}}}$ (eq 1)		$△{\text{G}}_{\text{Pi}}^{‡}$ (kcal/mol) eq 1	$\left[{(△{\text{G}}_{\text{Pi}}^{‡})}_{\text{WT}}\right]$− $\left[{(△{\text{G}}_{\text{Pi}}^{‡})}_{\text{Var}}\right]$ (kcal/mol)
Wild type		4.2 x 108	11.7
Q215A		2.3 x 107	10.0	1.7
Y217F		1.5 x 107	9.8	1.9
R235A		3.5 x 104	6.2	5.5
Q215A/Y217F		7.4 x 105	8.0	3.7
Q215A/ R235A		3300	4.8	6.9
Y217F /R235A		410	3.6	8.1
Q215A/Y217F/R235A		12	1.5	10.2

^aData from [54].