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. 2023 May 22;14:1183825. doi: 10.3389/fimmu.2023.1183825

Figure 3.

Figure 3

Lactate regulation of fibroblast and macrophage motility and IL-6 production. (A) Scratch test assay of synovial fibroblasts from RA patients (n=3 biological replicates). Cells were seeded for 24h and 48h in RPMI supplemented with 1% FBS and treated with or without sodium lactate (10 mM) +/- phroletin. (B) The perimeter (wound gap) of each scratch was measured with ImageJ software. The fold was calculated on Time 0 for each treatment (n=3, each in duplicate). (C) In vitro chemokinesis of monocytes-derived macrophages in response to CCL7 (300 ng/mL; n=6). Cells were cultured in medium containing sodium lactate (10 mM) or fibroblasts conditioned medium (10%) with or without phroletin (41 uM). Untreated macrophages (w/o CCL7, dotted line) were set to 100 (n=3, each in duplicate). (D) IL-6 ELISA from supernatants of fibroblasts and macrophages stimulated with TNFα (10 ng/ml) +/- lactate (n=3, each in duplicate). Untreated cells (dotted line) were set to 1. Data expressed are representative of n=3 biological replicates. Data are expressed as mean ± SEM. Anova test *p < 0.05; **p < 0.03; ***p < 0.01.