Table 1. Experimental Conditions Employed in “Multiwell Plate Plasma Digestion” Used to Examine the Edge Effect Intraplate Variation in High-Throughput Bottom-Up Proteomics.

Experiment	Multiwell Plate	Heater	Plate Lid	Other Changes
1	Waters QuanRecovery 700 μL Plates (Waters, Milford, USA)	Incubator Hood TH 30 (Edmund Buhler GmBH, Bodelshausen, Germany)	Corning clear polystyrene 96-well microplate lids (Thermo Fisher Scientific, Loughborough, UK) secured with SLS heat resistant laboratory tape (Scientific Laboratory Supplies, Nottingham, UK)
2	Waters QuanRecovery 700 μL Plates	Incubator Hood TH 30	Waters 96-well 7 mm round plug silicone/PTFE cap mat (Waters, Milford, USA) topped with the Corning lid sealed with tape
3	Waters QuanRecovery 700 μL Plates	Grant SUB6 Universal Water Bath (Grant Instruments, Cambridge, UK)	Waters silicone/PTFE cap mat topped with the Corning lid and sealed with tape
4	Waters QuanRecovery 700 μL Plates	Dry bath heater (Star Lab, Milton Keynes, UK) filled with Bath Armor heating beads (Appleton Woods Ltd., Birmingham, UK)	Waters silicone/PTFE cap mat topped with the Corning lid and sealed with tape
5	Eppendorf twin.tec semiskirted 250 μL 96-well plates (Eppendorf, Hamburg, Germany)	Thermo Scientific Hybaid PX2 thermal cycler (Thermo Fisher Scientific, Loughborough, UK)	Eppendorf Flat Microcap 8-Strips (Eppendorf, Hamburg, Germany)
6	Eppendorf twin.tec semiskirted 250 μL 96-well plates	Thermo Scientific Hybaid PX2 thermal cycler	Eppendorf Flat Microcap 8-Strips	Starting reagents adjusted to 70 μL AmBic and 5 μL bovine serum albumin (BSA, 0.1 mg/mL)