Table 1.

DRS metrics

Time post-degradation treatment (h)	Average RIN	N50 (bp)	Median aligned length (bp)	Full-length transcripts (%)	Transcripts within 25 bp of 5′ and 50 bp of 3′ (%)	Median coverage fraction per read	Median coverage fraction per isoform	Number of genes detected per 1M reads	Number of isoforms detected per 1M reads
0 (n = 3)	9.8	1378	773	25	20	0.68	0.53	14 727	33 502
0.5 (n = 2)	9.6	1460	774	24	19	0.67	0.52	14 849	34 387
1 (n = 2)	9.3	1203	697	20	17	0.57	0.42	14 942	34 431
3–4 (n = 3)	8.7	1009	628	19	17	0.53	0.38	14 733	35 164
6 (n = 3)	8.2	925.6	607	20	18	0.53	0.35	14 663	34 043
8 (n = 2)	7.25	777	518	16	16	0.47	0.29	14 459	33 806