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Comparing PCR-cDNA and DRS sequencing metrics
| Time post-degradation treatment (h) | Average RIN | N50 (bp) | Median aligned length (bp) | Full-length transcripts (%) | Median coverage fraction per read | Median coverage fraction per isoform | |
|---|---|---|---|---|---|---|---|
| Brain | 7.8 | 1730 | 904 | 21 | 0.52 | 0.44 | |
| PCR-cDNA | 0 (n = 2) | 9.8 | 1230 | 575 | 38 | 0.84 | 0.51 |
| 6 (n = 2) | 7.95 | 879 | 498 | 29 | 0.62 | 0.33 | |
| DRS | 0 (n = 2) | 9.8 | 1527 | 778 | 26 | 0.70 | 0.55 |
| 6 (n = 2) | 7.95 | 957 | 623 | 20 | 0.54 | 0.36 |
