Fig. 2.

Two treatments at 38 °C in the presence of d-valine exhibit selective toxicity towards HIBAT seedlings compared to Col-0 seedlings. Above: Experimental protocol involving two-week-old Col-0 and HIBAT seedlings cultivated at 22 °C in the presence of increasing concentrations of d-valine, followed by two consecutive 120-min heat treatments at 38 °C, with a 120-min interval at 22 °C. Below: Representative images of Col-0 and HIBAT seedling lawns grown on Petri dishes containing 0 mM, 20 mM, 25 mM, and 30 mM d-valine, without heat treatment (no HS) or following the two heat treatments (HS). HIBAT seedling mortality was observed six days after the heat treatments