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. 2023 Jun 8;19:56. doi: 10.1186/s13007-023-01033-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Effect of chilling and cold-shock on the expression of nLUC-DAO1 in HIBAT. On day 12, seedlings were subjected to different temperature treatments: cold shock (0 °C), chilling (4 °C to 16 °C), or maintained at room temperature (22 °C) for a duration of 2 h. Following the treatments, measurements were taken from cell extracts after 2 h of post-treatment at 22 °C. The data are presented as means ± standard deviation (n = 4). Statistical analysis using the Student's t-test showed no significant difference at 22 °C compared to all other conditions