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Transactivation potential of wild-type and mutated HNF4α in transient transfection experiments at saturation. The reporter constructs hHNF1(–325/+138)lucII (A) and H4-tk-luc (B) were co-transfected into HeLa cells with saturating amounts of expression vector (300 ng) encoding wild-type or the mutated HNF4α. Fold induction refers to the activity without any HNF4 derivative. The error bars indicate standard deviation of six determinations.
