Figure 4.

Saturation curves of transactivation potential of wild-type and mutated HNF4α in transfection assays. (A) Increasing amounts of the expression vectors encoding the HNF4α mutants as given were co-transfected with the reporter construct hHNF1(–325/+138)lucII. The dotted and the solid lines represent the activity of the mutant and the wild-type, respectively. (B) The expression vector encoding the HNF4α mutant D69A was co-transfected with either the Xenopus HNF1(–594/207) promoter construct or the rat apoAI (–1000/+14)lucI reporter. Fold induction refers to the activity without any HNF4 derivative and the error bars indicate standard deviation of six determinations.