Table 1.
Overview of compared test systems.
| Abbreviation | Manufacturer | Assay name | Method | Antigen | Isotype | Units |
|---|---|---|---|---|---|---|
| Wt-pVNT | Institute of virology, UHC | In-house pVNT | pVNT | spike protein, wt | All | ID50 |
| Om-pVNT | spike protein, BA.1 | |||||
| Wt-cPass | GenScript | cPass SARS-CoV-2 Neutralization Antibody Detection Kit |
sVNT using ELISA technique | RBD, wt | All | Inhibition% |
| Om-cPass | RBD, BA.1 | |||||
| Wt-QuantiVac | Euroimmun | Anti-SARS-CoV-2 QuantiVac ELISA (IgG) | ELISA | S1, wt | IgG | BAU/ml |
| Om-QuantiVac | Anti-SARS-CoV-2 Omicron ELISA (IgG) | S1, BA.1 | RU/ml | |||
| Wt-SeraSpot | Seramun | SeraSpot Anti-SARS-CoV-Z2 IgG | ELISA Spot test | RBD, wt | IgG | BAU/ml |
| Om-SeraSpot | RBD, BA.1 | RU/ml |
BAU, binding antibody units; ELISA, Enzyme-linked Immunosorbent Assay; ID50, 50% inhibitory dilution; IgG, immunoglobulin G; om, Omicron BA.1; pVNT, Pseudovirus neutralization assay; RBD, receptor-binding-domain; RU, relative units; S1, subunit 1 of spike protein; sVNT, Surrogate virus neutralization test; UHC, university hospital Cologne; wt, wild-type (refers to Wu01). The abbreviations Wt/Om in the first column refer to the antigens used in the test.