Table 3.
ELISA test performances with regard to pVNT results.
| Test comparison | At manufacturer's cut-off | At max. Youden index | At >99% specificity | |||||
|---|---|---|---|---|---|---|---|---|
| Cut-off | Sensitivity [%] | Specificity [%] | Cut-off | Sensitivity [%] | Specificity [%] | Cut-off | Sensitivity [%] | |
| A) Compared with Wt-pVNT | ||||||||
| Wt-cPass | ≥ 30% | 90.8 | 100 | > 30.4% | 90.8 | 100 | > 30.4% | 90.8 |
| Wt-QuantiVac | ≥ 35.2 BAU/ml | 98.8 | 72.2 | > 109.8 BAU/ml | 92.8 | 100 | > 109.8 BAU/ml | 92.8 |
| Wt-SeraSpot | ≥ 230 BAU/ml | 90.4 | 100 | > 180.5 BAU/ml | 93.6 | 100 | > 180.5 BAU/ml | 93.6 |
| B) Compared with Om-pVNT | ||||||||
| Wt-cPass | ≥ 30% | 99.1 | 69.6 | > 90.7% | 82.2 | 98.2 | > 98.5% | 63.9 |
| Wt-QuantiVac | ≥ 35.2 BAU/ml | 100 | 28.6 | > 389.4 BAU/ml | 90.6 | 85.7 | > 3062 BAU/ml | 29.6 |
| Wt-SeraSpot | ≥ 230 BAU/ml | 95.8 | 58.9 | > 281.0 BAU/ml | 90.6 | 66.1 | > 1278 BAU/ml | 16.4 |
| C) Compared with Om-pVNT | ||||||||
| Om-cPass | ≥ 30% | 51.2 | 100 | > 11.9% | 65.3 | 100 | > 11.9% | 65.3 |
| Om-QuantiVac | ≥ 11.0 RU/ml | 99.1 | 57.1 | > 32.3 RU/ml | 93.0 | 87.5 | > 206.8 RU/ml | 40.4 |
| Om-SeraSpot | ≥ 247 RU/ml | 99.5 | 55.4 | > 1139 RU/ml | 93.0 | 87.5 | > 4651 RU/ml | 32.9 |
BAU, binding antibody units; Om, Omicron BA.1; pVNT, Pseudovirus neutralization assay; RU, relative units; Wt, wild-type.