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. 2023 Jun 9;26(7):107085. doi: 10.1016/j.isci.2023.107085
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
W25 Valenzuela Nieto et al.27 N/A
W270 Valenzuela Nieto et al.27 N/A
NB3 Schoof et al.52 N/A
NB11 Schoof et al.52 N/A
NB20 Schoof et al.52 N/A
MR17 Li et al.62 N/A
SB14 N/A
SB42 N/A
SB4 N/A
Ty1 Hanke et al.31 N/A
H11 Huo et al.63 N/A
Nb against GFP Kubala et al.64 N/A
S309 Pinto et al.9 N/A
REGN10987 Hansen et al.10 N/A
REGN10933 Hansen et al.10 N/A
Ly-CoV555 Jones et al.11 N/A
CT-P59 Kim et al.12 N/A
CB6 Shi et al.8 N/A
DH1047 Martinez et al.13 N/A
5B3 Dang et al.65 N/A
C05 Ekiert et al.66 N/A
Anti-human IgG conjugated with an IRDye 800CW Millennium Scientific Cat#LCR-926-32232
Goat Anti-Human IgG Fc Highly Cross-Adsorbed Secondary Antibody Thermofisher Cat#A18829
Bacterial and virus strains
StellarTM Competent cells Takara Cat#636763
hCoV-19/Australia/QLD02/20200 This paper GISAID accession ID, EPI_ISL_407896
hCoV-19/Australia/QLD1517/2021 This paper GISAID accession ID EPI_ISL_944644
hCoV-19/Australia/QLD1520/2020 This paper GISAID accession ID EPI_ISL_968081
hCoV-19/Australia/QLD1893C/2021 This paper GISAID accession ID EPI_ISL_2433928
hCoV-19/Australia/NSW4318/2021 This paper GISAID accession ID EPI_ISL_1121976
hCoV-19/Australia/NSW4431/2021 This paper GISAID accession ID EPI_ISL_1494722
hCoV-19/Australia/NSW-RPAH-1933/2021 This paper GISAID accession ID EPI_ISL_6814922
Chemicals, peptides, and recombinant proteins
Spike proteins (Wuhan, Alpha, Beta, Gamma, Delta Lambda, BA.1, BA.2) This paper N/A
Critical commercial assays
KPL milk diluent/blocking solution concentrate SeraCare Cat#5140-0011
3,3,5,5′-Tetramethylbenzidine Thermofisher Cat#002023
Experimental models: Cell lines
ExpiCHO-STMcells Thermofisher Cat#29127
Flp-In™ T-REx™ 293 cells Thermofisher Cat#R78007
HEK293T ATCC CRL-3216
VeroE6 ATCC CRL-1586
VeroE6-TMPRSS2 This paper N/A
VeroE6-ACE2-TMPRSS2 BEI resource NR-54970
Experimental models: Organisms/strains
Transgenic hACE2 mice (2B6.Cg-Tg(K18-ACE2)2Prlmn/J) Jackson laboratory RRID:IMSR_JAX:034860
Oligonucleotides
primers for SARS-CoV-2 N gene Forward 5′- TTACAAACATTGGCCGCAAA-3′ This paper N/A
primers for SARS-CoV-2 N gene Reverse 5′-GCGCGACATTCCGAAGAA-3′ This paper N/A
primers for HPRT1 Forward 5′- GTTGGATACAGGCCAGACTTTGTTG-3′ This paper N/A
primers for HPRT1 Reverse 5′-GAGGGTAGGCTGGCCTATTGGCT-3′ This paper N/A
Software and algorithms
GraphPad Prism 8.01 GraphPad Software Inc. https://www.graphpad.com/scientific-software/prism/"
https://www.graphpad.com/scientific-software/prism/
MotionCorr2 Zheng et al.67 N/A
CTFFind4 Rohou et al.68 N/A
Relion 3.0.7 Zivanov et al.69 N/A
Relion 3.1.3 Zivanov et al.69 N/A
cryoSPARC 3.3.1 Punjani et al.70 N/A
AlphaFold2 Jumper et al.,71 Mirdita et al.33 N/A
Coot 0.9.5 Emsley et al.72 N/A
Phenix 1.19.2–4158 Liebschner et al.73 N/A
ChimeraX Petterson et al.84 N/A
Biacore Insight Evaluation Cytiva https://www.cytivalifesciences.com/en/us/shop/protein-analysis/spr-label-free-analysis/software/biacore-insight-evaluation-software-p-23528