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. 2023 Jun 8;61(1):886–896. doi: 10.1080/13880209.2023.2212700

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 2. — Met inhibited ferroptosis in the OGD/R-induced H9c2 cells. (A) CCK-8 assay was executed to monitor the viability of H9c2 cells. (B, C) The expression of PTGS2 mRNA and protein in H9c2 cells was explored by qRT-PCR and Western blot. (D) DCFH-DA staining of H9c2 cells was utilized for the detection of the intracellular lipid ROS level. (E) JC-1 staining was performed to evaluate the mitochondrial damage in H9c2 cells. (F, G) The levels of non-heme iron and MDA in H9c2 cells was monitored by ELISA. **p < 0.01 and ***p < 0.001 vs. OGD/R group.