Table 2.
Primer information.
| Primer Names | Primer Sequence (5′→3′) * | Product Length (bp) | Tm (T/°C) | Purpose |
|---|---|---|---|---|
| CXCL17 | F: 5′ CCTGTTGCTGCCACTAATGC 3′ R: 5′ GTGATGTCTTCGGTGTCTGGT 3′ |
250 | 60 | qPCR |
| CXCL17 | F: 5′ CTAGAATTCTGATGAAGGTTCTAATCTCTTCCC 3′ 1 R: 5′ CGGGGTACCACTAAGGGCAGAACAAAGCTT 3′ |
357 | 60 | plasmid construction |
| UXT | F: 5′ GCAAGTGGATTTGGGCTGTAAC 3′ R: 5′ ATGGAGTCCTTGGTGAGGTTGT 3′ |
180 | 60 | qPCR |
| CCNE1 | F: 5′ CTCCCTGATTCCCACACCTG 3′ R: 5′ CATAAGATGCTTGTCCCTCA3′ |
193 | 60 | qPCR |
| PCNA | F: 5′ AGTGGAGAACTTGGAAATGGAA 3′ R: 5′ GAGACAGTGGAGTGGCTTTTGT 3′ |
154 | 60 | qPCR |
| CCND1 | F: 5′ TGAACTACCTGGACCGCT 3′ R: 5′ CAGGTTCCACTTGAGTTTGT 3′ |
212 | 60 | qPCR |
| CDK2 | F: 5′ GCCAGGAGTTACTTCTATGC 3′ R: 5′ TGGAAGAAAGGGTGAGCC 3′ |
180 | 60 | qPCR |
* F: Sense primer; R: Antisense primer. 1 The double underlined lines are restriction sites, and the single underlined are bases protecting the restriction sites.