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. 2023 Jun 2;13(11):1857. doi: 10.3390/ani13111857

Table 4.

Comparison of animal performance, diet, and enteric methane emission data from studies with different methods to quantify enteric methane emission in lactating dairy cows.

Item 1 Method 2 p-Value 3
Respiration Chamber SF6 GreenFeed® Other
Animal
    Metabolic weight (BW0.75), kg 128.98 a ± 1.11
(n = 188)
125.00 b ± 1.11
(n = 114)
131.46 a ± 1.44
(n = 52)
134.16 a ± 3.28
(n = 8)
<0.01
    Milk yield (MY), kg/day 31.82 b ± 0.91
(n = 192)
29.63 c ± 0.91
(n = 132)
35.46 a ± 1.23
(n = 52)
33.12 abc ± 2.81
(n = 8)
<0.01
    Milk protein, g/kg 32.94 a ± 0.19
(n =161)
31.99 ab ± 0.23
(n = 105)
32.03 ac ± 0.38
(n = 40)
34.85 ac ± 1.69
(n = 2)
<0.01
    Milk fat, g/kg 39.91 ab ± 0.58
(n = 169)
38.25 a ± 0.61
(n = 111)
38.99 ab ± 0.85
(n = 40)
43.01 ab ± 3.52
(n = 2)
0.04
    DMI, kg/day 21.06 b ± 0.41
(n = 191)
20.76 b ± 0.43
(n = 125)
23.42 a ± 0.62
(n = 40)
23.25 ab ± 1.72
(n = 4)
<0.01
Diet
    Forage in diet, g/kg DM 596.56 a ± 12.33
(n = 178)
543.79 b ± 14.92
(n = 64)
569.28 ab ± 17.78
(n = 42)
623.83 a ± 38.94
(n = 8)
<0.01
    NDF, g/kg DM 346.53 ± 7.32
(n = 163)
344.78 ± 8.02
(n = 81)
344.11 ± 10.35
(n = 42)
397.69 ± 25.40
(n = 6)
0.23
    Crude protein, g/kg DM 166.70 ± 1.37
(n = 182)
165.66 ± 2.25
(n = 68)
171.20 ± 2.79
(n = 44)
159.00 ± 7.56
(n = 6)
0.29
    Ether extract, g/kg DM 42.58 a ± 1.52
(n = 134)
36.09 b ± 2.45
(n = 35)
34.24 b ± 2.90
(n = 25)
28.02 b ± 6.97
(n = 4)
<0.01
    Starch, g/kg DM 191.96 ± 9.11
(n = 113)
204.93 ± 11.97
(n = 48)
211.56 ± 16.77
(n = 23)
164.38 ± 36.65
(n = 4)
0.42
    OM digestibility, g/kg 709.81 a ± 7.57
(n = 105)
674.03 b ± 8.17
(n = 44)
691.87 ab ± 10.70
(n = 26)
693.48 ab ± 32.24
(n = 2)
<0.01
Methane Enteric Emission
    Production, g/cow/day 445.3 a ± 10.61
(n = 218)
406.0 b ± 10.75
(n = 142)
470.0 a ± 14.52
(n = 53)
458.0 a ± 38.32
(n = 6)
<0.01
    SEM, g/cow/day 22.28 b ± 1.97
(n = 207)
27.55 a ± 2.03
(n = 136)
22.32 b ± 2.92
(n = 45)
13.42 b ± 7.48
(n = 6)
0.03
    Yield, g/kg DMI 21.20 ± 0.47
(n = 191)
20.19 ± 0.50
(n = 125)
20.55 ± 0.77
(n = 40)
17.89 ± 2.23
(n = 4)
0.13
    Intensity, g/kg MY 14.53 ± 0.36
(n = 192)
15.38 ± 0.44
(n = 132)
14.35 ± 0.69
(n = 52)
13.53 ± 2.04
(n = 6)
0.38

1 DMI = dry matter intake; NDF = neutral detergent fiber; OM = organic matter; SEM = standard error of mean. 2 Least squares mean ± standard error (n = observation). 3 F-test to compare studies with different enteric methane emission methods. a–c Means values in the same row with different superscripts differ (p < 0.01).